One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification prools. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.
Table of Contents1 Affinity-Based Separation: An Overview.- 2 Affinity Chromatography.- 3 Expanded Bed Affinity Chromatography.- 4 Perfusion Affinity Chromatography for Rapid Antibody Separations.- 5 Affinity-Based Interactions on Disks for Fast Analysis, Isolation and Conversion of Biomolecules.- 6 Sugars as Affinity Ligands.- 7 Macroaffinity Ligands in Bioseparation.- 8 Cellulose-Binding Fusion Proteins.- 9 Two-Phase Affinity Partitioning of Animal Cells: Implications of Multipoint Interactions.- 10 Affinity-Based Reverse Micellar Separations.- 11 Biosensors: An Emerging Tool in Bioanalytical Research -A Kinetic Analysis of the Analyte-Receptor Interactions in Biosensors.- Guide to Solutions.- Guide to Enzymes.- Guide to Protocols.- Troubleshooting Guide.