Phosphodiesterase Methods and Protocols

Research leaders in the PDE field describe new concepts and techniques for investigating the role of PDEs in orchestrating normal and pathophysiological responses. Presented in step—by—step detail, these readily reproducible methods allow the measurement of cyclic nucleotide variations in living cells, as well as their visualization in a spatio—temporal manner, the localization and characterization of their activities in tissues and living cells, and the assessment of targeted PDEs in creat...