Ras Signaling: Methods and Protocols

Ras Signaling: Methods and Protocols

Paperback(Softcover reprint of the original 1st ed. 2014)

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Product Details

ISBN-13: 9781493960545
Publisher: Springer-Verlag New York, LLC
Publication date: 08/27/2016
Series: Methods in Molecular Biology , #1120
Edition description: Softcover reprint of the original 1st ed. 2014
Pages: 425
Product dimensions: 7.01(w) x 10.00(h) x 0.04(d)

Table of Contents

1. The Ras Superfamily of Small GTPases: The Unlocked Secrets

Luca Goitre, Eliana Trapani, Lorenza Trabalzini, and Saverio Francesco Retta

2. A Novel Method for the Production of Fully-Modified K-Ras 4B

Tanmay S. Chavan, James O. Meyer, Luke Chisholm, Malgorzata Dobosz, and Vadim Gaponenko

3. Metabolic Labeling of Ras with Tritiated Palmitate to Monitor Palmitoylation and Depalmitoylation

Frederick D. Tsai, Joseph P. Wynne, Ian M. Ahearn, and Mark R. Philips

4. Ubiquitination of Rac1 by Inhibitors of Apoptosis (IAPs)

Tripat Kaur Oberoi-Khanuja and Krishnaraj Rajalingam

5. Ras GTPases are Both Regulators and Effectors of Redox Agents

Elisa Ferro, Luca Goitre, Eva Baldini, Saverio Francesco Retta, and Lorenza Trabalzini

6. Biophysical and Proteomic Characterization Strategies for Cysteine Modifications in Ras GTPases

G. Aaron Hobbs, Harsha P. Gunawardena, and Sharon L. Campbell

7. Use of the Yeast Two-Hybrid Technology to Isolate Molecular Interactions of Ras GTPases

Elisa Ferro, Eva Baldini, and Lorenza Trabalzini

8. Screening for MAPK Modulators Using an In-Cell Western Assay

Simon Schnaiter, Beatrix Fürst, Johannes Neu, Frigyes Wáczek, László Őrfi, György Kéri, Lukas A. Huber, and Winfried Wunderlich

9. Behavioral Methods for the Study of the Ras-ERK Pathway in Memory Formation and Consolidation: Passive Avoidance and Novel Object Recognition Tests

Raffaele d’Isa, Riccardo Brambilla, and Stefania Fasano

10. Functional Phosphoproteomics of Oncogenic KRAS Signaling

Putty-Reddy Sudhir and Jeou-Yuan Chen

11. Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets

Gianni Francesco Guidetti and Mauro Torti

12. Combined Pulldown and Time-Lapse Microscopy Studies for Determining the Role of Rap1 in the Crosstalk between Integrins and Cadherins

Luca Goitre, Alessandro Morina, and Saverio Francesco Retta

13. Fluorescence Microscopy Study of Rap1 Subcellular Localization

Luca Goitre, Valentina Cutano, and Saverio Francesco Retta

14. AnIn VitroSystem to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1

David F. Kashatus

15. Analysis of the Rit Subfamily GTPase-Mediated Signaling and Neuronal Differentiation and Survival

Geng-Xian Shi and Catherine N. Kaminski

16. Immunofluorescence Methods in Studies of the GTPase RAN and Its Effectors in Interphase and in Mitotic Cells

Giulia Guarguaglini, Valeria de Turris, and Patrizia Lavia

17. High Resolution Scanning Electron Microscopy for the Imaging of Nuclear Pore Complexes and Ran-Mediated Transport

Lihi Shaulov, Boris Fichtman, and Amnon Harel

18. Effector Recruitment Method to Study Spatially Regulated Activation of Ras and Rho GTPases

Lauren P. Huff, Molly J. DeCristo, and Adrienne D. Cox

19. Real-Time Visualization and Quantification of Native Ras Activation in Single Living Cells

Christoph Biskup and Ignacio Rubio

20. Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis

Camilo Guzmán, Maja Šolman, and Daniel Abankwa

21. Analyzing the Roles of Rho GTPases in Cancer Cell Migration with a Live Cell Imaging 3D-Morphology-Based Assay

Audrey Colomba and Anne J. Ridley

22. Analysis of Rho-GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy

Annica K.B. Gad, Daniel Rönnlund, Jerker Widengren, and Pontus Aspenström

23. Yeast as a Model for Ras Signaling

Renata Tisi, Fiorella Belotti, and Enzo Martegani

24. Methods to Study the Ras2 Protein Activation State and the Subcellular Localization of Ras-GTP in Saccharomyces cerevisiae

Sonia Colombo and Enzo Martegani

25. RAS Proteins Signaling in the Early Metazoan Dictyostelium discoideum

Enrico Bracco and Barbara Pergolizzi

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