Restriction Landmark Genomic Scanning (RLGS) is a new multiplex method for simultaneous analysis of over 3,000 genome loci. Written by the inventor of RLGS, Yoshihide Hayashizaki, and his co-workers, this is the first manual on this method. RLGS is a powerful technique with enormous potential for biological (animal and plant sciences), and biomedical research. Yielding results much faster than conventional techniques, RLGS is particularly suited to the identification of the chromosomal location of the genes implicated in inherited diseases, and for the genetic disturbances present in cancerous tissue.
Table of Contents1 Concept of Genome Scanning.- 2 Principle of RLGS.- 3 Protocols for RLGS Gel Production.- 4 RLGS Spot Cloning.- 5 RLGS Spot Mapping Method.- 6 Application of RLGS to Screening Endogeneously Imprinted Genes.- 7 Systematic Detection of DNA Alteration in Cancer Tissue.- 8 RLCS, Restriction Landmark cDNA Scanning.- 9 Concluding Remarks.- Acknowledgments.- Abbreviations.- List of Suppliers.