Split Inteins: Methods and Protocols

This volume focuses on applications of split inteins, and the progress that has been made in the past 5 years on discovery and engineering of fast and more efficient split inteins. The first few chapters in Split Inteins: Methods and Prools explore new techniques on how to use split inteins for affinity purification of overproduced proteins, and split—intein based technologies to prepare cyclic peptides and proteins. The next few chapters discuss semisynthetic protein trans—splicing using o...