The Myc Gene: Methods and Protocols

by Laura Soucek (Editor), Jonathan Whitfield (Editor)

Overview

This second edition provides new and updated chapters detailing recent advances in MYC research and current techniques. Chapters guide readers through protocols on how to express and purify MYC protein, X-ray crystallography, NMR, techniques to study how MYC is modified, apoptosis, senescence, proliferation, metabolic changes, translation, tumorigenesis, reprogramming, and clinical application of MYC studies.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, The Myc Gene: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Product Details

ISBN-13:	9781071614785
Publisher:	Springer US
Publication date:	05/23/2021
Series:	Methods in Molecular Biology , #2318
Edition description:	2nd ed. 2021
Pages:	349
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

The Myc world within Reach.- Methods for the Expression, Purification, Preparation and Biophysical Characterization of Constructs of the c-Myc and Max b-HLH-LZs.- Analyzing Myc in Cell Transformation and Evolution.- Identifying Myc Interactors.- Detection of c-Myc Protein-Protein Interactions And Phosphorylation Status by Immunoprecipitation.- An Efficient Way of Studying Protein–Protein Interactions Involving HIF-α, c-Myc, and Sp1.- Methods for Determining Myc-induced Apoptosis.- Methods to Study Myc-regulated Cellular Senescence.- Chromatin Immunoprecipitation Assays for Myc and N-Myc.- Methods to Quantify microRNAs in the Myc Gene Network for Posttranscriptional Gene Repression.- Genome-wide Analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing.- A High Throughput siRNA Screening Platform to Identify MYC Synthetic Lethal Genes as Candidate Therapeutic Targets.- Investigating Myc-dependent Translational Regulation in Normal and Cancer Cells.- Studying Myc’s Role in Metabolism Regulation.- Generation of a Tetracycline Regulated Mouse Model of MYC-induced T-cell Acute Lymphoblastic Leukemia.- Methods to Assess Myc Function in Intestinal Homeostasis, Regeneration and Tumourigenesis.- Detection of c-myc Amplification in Formalin-fixed Paraffin-Embedded Tumor Tissue by Chromogenic in situ Hybridization (CIHS).- Cell-Based Methods for the Identification of MYC-Inhibitory Small Molecules.