The Ubiquitin Code

This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide readers through ubiquitin chain complexity, methods to generate non—hydrolysable ubiquitin—NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase—mediated ubiquitylation in vitro, a RNAi—based screening to identify DUBs, a Yuh1 activity assay, a pull—down to isolate K48 and K63 chains using specific nanobodies, identification of N...